Skip the Alignment: Degenerate, Multiplex Primer and Probe Design Using K-mer Matching Instead of Alignments | PLOS ONE
![Minimum degenerated sequence generation. (a) Diagram of the general... | Download Scientific Diagram Minimum degenerated sequence generation. (a) Diagram of the general... | Download Scientific Diagram](https://www.researchgate.net/publication/236085320/figure/fig1/AS:1089002807529473@1636649910313/Minimum-degenerated-sequence-generation-a-Diagram-of-the-general-strategy-used-b.jpg)
Minimum degenerated sequence generation. (a) Diagram of the general... | Download Scientific Diagram
![Table 1 from Construction of "small-intelligent" focused mutagenesis libraries using well-designed combinatorial degenerate primers. | Semantic Scholar Table 1 from Construction of "small-intelligent" focused mutagenesis libraries using well-designed combinatorial degenerate primers. | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/aa9bb70f962949fabc8fb7c03a3583f13616188c/5-Table1-1.png)
Table 1 from Construction of "small-intelligent" focused mutagenesis libraries using well-designed combinatorial degenerate primers. | Semantic Scholar
![SOLVED: These primers are known as “degenerate” primers because there is variation in the bases present at certain sites. Why might it be advantageous to include degenerate sites in the primers? SOLVED: These primers are known as “degenerate” primers because there is variation in the bases present at certain sites. Why might it be advantageous to include degenerate sites in the primers?](https://cdn.numerade.com/ask_previews/5371ad5c-c4a3-46d6-b2c4-4729ecf0a8ba_large.jpg)
SOLVED: These primers are known as “degenerate” primers because there is variation in the bases present at certain sites. Why might it be advantageous to include degenerate sites in the primers?
![Table 2.1 from An Iterative Beam Search Algorithm for Degenerate Primer Selection | Semantic Scholar Table 2.1 from An Iterative Beam Search Algorithm for Degenerate Primer Selection | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/cc4eb7a97238ca9492f0bb9f1a58202a3b2fca95/20-Table2.1-1.png)
Table 2.1 from An Iterative Beam Search Algorithm for Degenerate Primer Selection | Semantic Scholar
![PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens | Communications Biology PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens | Communications Biology](https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fs42003-021-01751-9/MediaObjects/42003_2021_1751_Fig1_HTML.png)
PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens | Communications Biology
![IJERPH | Free Full-Text | ARDEP, a Rapid Degenerate Primer Design Pipeline Based on k-mers for Amplicon Microbiome Studies IJERPH | Free Full-Text | ARDEP, a Rapid Degenerate Primer Design Pipeline Based on k-mers for Amplicon Microbiome Studies](https://pub.mdpi-res.com/ijerph/ijerph-17-05958/article_deploy/html/images/ijerph-17-05958-g001-550.jpg?1598582731)
IJERPH | Free Full-Text | ARDEP, a Rapid Degenerate Primer Design Pipeline Based on k-mers for Amplicon Microbiome Studies
![Design of degenerate PCR primers. (A) Alignment of six mammalian L1... | Download Scientific Diagram Design of degenerate PCR primers. (A) Alignment of six mammalian L1... | Download Scientific Diagram](https://www.researchgate.net/publication/12203032/figure/fig2/AS:667181956222987@1536079982500/Design-of-degenerate-PCR-primers-A-Alignment-of-six-mammalian-L1-reverse.png)
Design of degenerate PCR primers. (A) Alignment of six mammalian L1... | Download Scientific Diagram
![PDF] Degenerate Primer Design Using Computational Tools Computational Molecular Biology | Semantic Scholar PDF] Degenerate Primer Design Using Computational Tools Computational Molecular Biology | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/89cb6d6c927e29073c9fd66ccfab088d2f9895ff/10-Figure4-1.png)
PDF] Degenerate Primer Design Using Computational Tools Computational Molecular Biology | Semantic Scholar
![Development of RT-PCR degenerate primers for the detection of two mandariviruses infecting citrus cultivars in India - ScienceDirect Development of RT-PCR degenerate primers for the detection of two mandariviruses infecting citrus cultivars in India - ScienceDirect](https://ars.els-cdn.com/content/image/1-s2.0-S0166093418303501-gr1.jpg)
Development of RT-PCR degenerate primers for the detection of two mandariviruses infecting citrus cultivars in India - ScienceDirect
![In silico design and validation of a highly degenerate primer pair: a systematic approach | Journal of Genetic Engineering and Biotechnology | Full Text In silico design and validation of a highly degenerate primer pair: a systematic approach | Journal of Genetic Engineering and Biotechnology | Full Text](https://media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs43141-020-00086-y/MediaObjects/43141_2020_86_Fig10_HTML.png)
In silico design and validation of a highly degenerate primer pair: a systematic approach | Journal of Genetic Engineering and Biotechnology | Full Text
![Designing multiple degenerate primers via consecutive pairwise alignments | BMC Bioinformatics | Full Text Designing multiple degenerate primers via consecutive pairwise alignments | BMC Bioinformatics | Full Text](https://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1471-2105-9-55/MediaObjects/12859_2007_Article_2040_Fig1_HTML.jpg)